Single Particles in IMAGIC
Image processing and image analysis of single particles
Description
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Standard image manipulations, cutting of images, blow up, rotations, filtering (high-pass, low-pass, etc.), arithmetic operations, Fourier transformations, statistics, complex images, display and plot, etc. |
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CTF correction | |
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Particle picking and storage within one (huge) file, pre-treatment of images, masking, normalisation, band-pass filtering, etc. | |
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Correlations (cross correlation, mutual correlation), (multi-reference) alignment, rotational and translational alignment, rotational average, etc. | |
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(Intra-class) averaging, resolution criteria, etc. | |
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Multivariate statistical analysis, eigenvalue-eigenvector calculations, data reduction, classification, MSA maps, etc. | |
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Determination of Euler angles and 3-D reconstruction | |
Relevant Pointers
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The IMAGIC image processing and analysis system: |
| Single Particles | |
| 3-D Processing and Reconstruction | |
| 2-D Crystals | |
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Morphology
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IMAGIC EM Package |
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GISP: The GUI oriented IMAGIC Single Particles Analysis |
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IMAGIC Shared Resources
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em2em: 3DEM Image Conversion Program |
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FSC: Program to calculate the Fourier Shell Correlation
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References
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