em2em: 3DEM Conversion Program
Description | Download | ManualDescription
Program to convert images (2-D images and 3-D volumes) from/to formats typically used in 3-D electron microscopy (3DEM).
FORMATS CURRENTLY SUPPORTED:
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BROOKHAVEN STEM | A format used to store STEM images (Import only) |
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CCP4 | Crystallography community format |
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DATA ONLY | Raw images, no header available |
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DIG. MICROGRAPH 2 | Gatan's dm2 format |
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EM | MPI für Biochemie, Martinsried, Germany |
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FEI MRC | FEI's MRC files with extended header |
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FEI MRCS | FEI's MRCS files (stacked MRC file) |
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FEI Raw Image | FEI's raw image format |
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FORMATTED | ASCII formatted files |
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IMAGIC | Image Science, Berlin, Germany |
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JPEG | Coloured/grey scale to grey scale images |
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KONTRON | Kontron image format |
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MDPP | NYU-MC, Ross Smith |
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MEDIPIX | Raw MEDIPIX image format |
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MRC | MRC/LMB, Richard Henderson & Tony Crowther, others |
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OFFSET | Skip a specified number of (header) bytes |
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PIF | Purdue Ariality, Tim Baker |
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PGM | Portable greymap format (P2 and P5) |
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POSTSCRIPT | Adobe Postscript (export only) |
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RAWIV/VOLUMETRIC | The rawiv data format is used to represent3D volumetric data of scalar fields defined on a regular grid |
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RGB_TIFF | The red/green/blue parts of a coloured TIFF image are imported/exported as single (greyscale) images |
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SHF | GATAN's simple header file format |
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SITUS | The map format of Willy Wrigger's docking program Situs |
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SPIDER | Albany, Joachim Frank |
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SUPRIM | J.P. Schroeter and P. Bretaudiere, Also used in PHOELIX (B. Carragher, M. Whittaker & R. Milligan) |
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TIA (EMISPEC) | The EMISPEC format used bei FEI (TECNAI/TITAN) |
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TIFF | Tagged image format |
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TVIPS | Image formats used by the TVIPS (Tietz Video and Image Processing Systems) company, Germany. (The two formats used are EM and TIFF with a specific tag) |
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X-PLOR | A system for X-ray crystallography and NMR (Import only) |
CORRECTION FOR BYTE SWAP:
Images from different workstations and operating systems can be converted automatically, i.e.
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| UNIX / Linux |
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| Mac OS X |
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| MS Windows |
CONVERSION OF MULTIPLE IMAGES:
Series and stacks of image/volume files can be converted in one ste.p
EASE OF USE:
There is a GUI windows application of em2em (guiEM2EM). and, if wanted, a line-by-line version running in a terminal/command window.
All questions and options are asked by em2em before the conversion starts.
INTERACTIVE HELP:
"?" is a legitimate answer to every question asked by em2em. "?" provides context sensitive help. The program also suggests default answers.
Interested?
Groups running IMAGIC or having signed a Shared Resources agreement will find em2em within their IMAGIC system (IMAGIC / guiIMAGIC). You can call the command by either giving EM2EM or IMPORT-EXPORT.
If you do not use IMAGIC you can download your FREE EM2EM (guiEM2EM) copy here.
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