Single Particles in IMAGIC

Image processing and image analysis of single particles



Standard image manipulations, cutting of images, blow up, rotations, filtering (high-pass, low-pass, etc.), arithmetic operations, Fourier transformations, statistics, complex images, display and plot, etc. Single Particles
CTF correction

Particle picking and storage within one (huge) file, pre-treatment of images, masking, normalisation, band-pass filtering, etc.
Correlations (cross correlation, mutual correlation), (multi-reference) alignment, rotational and translational alignment, rotational average, etc.
(Intra-class) averaging, resolution criteria, etc.
Multivariate statistical analysis, eigenvalue-eigenvector calculations, data reduction, classification, MSA maps, etc.
Determination of Euler angles and 3-D reconstruction

Relevant Pointers

The IMAGIC image processing and analysis system:
  Single Particles
  3-D Processing and Reconstruction
  2-D Crystals


IMAGIC Shared Resources

em2em: 3DEM Image Conversion Program

FSC: Program to calculate the Fourier Shell Correlation


Images used with friendly permission of Dr Marin van Heel Imperial College, UK
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